The Ajayi Crowther University (ACU), Oyo, is located on the Ibadan-Oyo-Ilorin road in Atiba Local Government Area (LGA) of Oyo State. The Campus can be reached within one hour by road from Ibadan and Ogbomosho, in about one-and-half hours from Ilorin and Ile-Ife and in about two hours from Lagos, all of which are University Towns. By virtue of its location, ACU has great opportunities for both Academic and Social interactions with many University Towns in its region.

Oyo, now a University Town, is cosmopolitan due to the traditional hospitality of the people and this diversity in population will rub off on the ACU campus like the erstwhile St. Andrews College which had students from all over the country and the former St. Andrews College of Education, a State College of Education, which had students from 14 of the 30 states of the federation in 1995.

The University has Two Campuses: The St. Andrews Campus and The Offa-Meta Campus. The old St. Andrews College campus is located on a gentle slope and has a land area of 37.02 hectares. By using the site of the old St. Andrews College which was established in Oyo in 1896, this campus of the University is now completely within the built-up area of Oyo Town. This has afforded the University the opportunity of sharing municipal services and utilities with the town. It also makes the campus and the town easily accessible from each other. In addition to the St. Andrews College Campus, additional 109 hectares had been acquired about 2km from the St. Andrews Campus on Oyo-Ogbomoso Road, thereby bringing the total land holding of the University to 146.02 Hectares.